PETerna® Technical Bulletin 

 

The broad benefits of colostrum to promote health in a variety of species at all stages of life are ascribed to immunological and metabolic components present in bovine colostrum.  To our knowledge, PETerna® is the only colostrum product extensively tested to confirm the presence and levels of seven key metabolic, immunological, and additional factors, plus more than fifty other naturally occurring beneficial ingredients.    Our ability to test for these components allowed us to develop proprietary (patent pending) colostrum processing techniques to preserve several key components that have been linked to beneficial health effects.

 

Veterinarians have long recognized that colostrum is necessary for the health of the newborn. The colostral transfer of maternal antibodies (immunoglobulins) from mother to infant during the first hours of life has been established as essential for survival in many species. In addition to antibodies, colostrum is rich in nutrients and other components that have documented beneficial effects in tissue and organ growth and development, aiding healing and combating aging. (Playford et. al. 2000).

 

It has been recognized that the benefits of colostrum are not confined to only newborns. The following is a partial list of reported benefits of bovine colostrum in older animals and humans:

 

Bovine colostrum and protein fractions from colostrum have been shown to be effective for treating and preventing infectious diseases in human and various animal species beyond the neonatal period (Reviewed in van Hooijdonk, A.C.M. et. al 2000, Tacket C. et. al. (1989) The Lancet 23: 709 – 712, Rump J. et. al. (1992) Clin. Invest. 70: 588 – 594).

 

Factors isolated from colostrum accelerate healing, balance blood sugar, reduce insulin need, and increase muscle and bone growth, while reducing fat (Reviewed in Pakkanen R. and Alto J. (1997) Int. Dairy J.: 285 – 297)

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Colostrum has been demonstrated to enhance athletic performance and increase lean body mass in humans. Journal Sports Sciences 2003 21:577-88,  (Mero et. al. (1997) J. Appl. Physiol. 83: 1144 – 1151; Buckley, J. D. et. al. (2000) Asian Aust. J. Anim. Sci. 13: 312 – 315; Sanders, A. J. and Van Gammeren, D. (2000) Nutrition 3: 343 – 247

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Colostrum has been shown to aid in recovery from Rotavirus induced diarrhea in children (J Pediatr Gastrenterol Nutr 23(3) 207-12)

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Colostrum has shown decreased numbers of upper respiratory tract infections in men  (European J. Nutrition 2003;43:228-32)

 

Recovery from Cryptospordia induced diarrhea in AIDS patients (GastroEnterol.1990;98;486-489)

 

Use of colostrum has shown increased immune responses. (Microbiol. Immunol. 2003;47:461-468)

 

Colostrum reduces damage to the gastrointestinal tract due to the digestion of nonsteroidal anti-inflammatory drugs (Playford, R. J. et. al. (1999) Gut 44: 653 – 658 and Playford, R. J. et. al. (2001).

 

Colostrum also reduces the detrimental effects attributable to microbial infection, decreases fat metabolism in old animals, reduces allergies and autoimmune diseases, heart disease, athletic stress, leaky gut syndrome, cancer, diabetes, and increases wound healing (Reviewed in Rona, Z. P. (1998) Am. J. Natural Med.)

 

Colostrum improves liver function by reducing the effects of toxic metabolites on the liver and provides desirable protein-rich nutrition for people suffering from a variety of liver disorders (U.S. Pat. No. 5, 710, 132, 1996, Use of bovine colostral milk as a preparation for the protection of the liver).

 

Colostrum strengthens the natural defense systems in the mouth resulting in improved healing of gum disorders (Canadian Pat. No. 2, 175, 319) and aids in the prevention of gingivitis and periodontal disease**  Oral Microbiol Immunol 2002,17:9-15 J. Periodontol. 1994;65:1158-1168** Naturally occurring disease in beagles

 

PETerna® is a LACT Innovation

 

Renewed interest in the broad benefits of colostrum to promote health in a variety of species at all stages of life prompted veterinarians and scientists at LACT (Laboratory for Advanced Colostrum Technologies) to investigate the possibility of developing a “complete colostrum” product for pets. To this end, they have developed colostrum processing techniques to preserve several key components that have been linked to beneficial health effects, as well as analytical techniques to measure their concentration. These assays allow our quality control staff to ensure that PETerna® contains defined levels of seven key metabolic, immunological, and other factors as summarized below:

 

1. Metabolic Factors - Colostrum contains high levels of numerous growth and cell differentiating factors such as Insulin-like Growth Factor-1 (IGF-1), Epidermal Growth Factor (EGF), Transforming Growth Factor Beta (TGF Beta) and Betacellulin.

 

Insulin-Like Growth Factor (IGF-1) has been shown to be an important pathway by which growth hormone exerts its metabolic and growth promoting effects. IGF-1 may be beneficial in combating the metabolic effects of aging. This growth factor promotes cellular mitogenesis and differentiation, enhances cellular glucose uptake, and stimulates the synthesis of proteins. (Review by Pakkanen, R. and Aalto, J., 2000). It also has anti-obesity effects.

Epidermal Growth Factor (EGF) promotes epithelial cell growth and differentiation (Carpenter, G. 1980).

Transforming Growth Factor Beta (TGF Beta) promotes healing of gastric ulcers (Am J. Physiol. Gastrointest. Liver Physiol.  2000;G105-G112)

Betacellulin shows reduced damage to the gut when taking NSAIDs (Non-steroidal anti-inflammatory drugs)  (indomethacin) Clin Sci 2001; 100:627-33

 

 

2.  Immunological and Antimicrobial Factors - Immunoglobulins, lactoferrin, and lysosymes are the best understood beneficial agents present in colostrum.  These and other antimicrobial agents present in colostrum work in concert to enhance defenses against microbial attacks of the gastrointestinal tract (Review by Pakkanen R. and Aalto J., 1997; Shah, N. P., 2000; van Hooijdonk A.C.M. et. al., 2000; and Steijns J.M. and van Hooijdonk A.C.M., 2000).  The concentration of immunoglobulins in colostrum is several times higher than that in serum.  Colostrum immunoglobulins are not absorbed into the systemic circulation past the neonatal period. Antimicrobial proteins such as immunoglobulins, lysozymes, and lactoferrin resist the digestive enzymes and low pH of the upper gastrointestinal tract due to the presence of trypsin inhibitor in PETerna®, thus allowing these proteins to remain biologically active to help combat enteric infections.

 

3.  Nutritional Factors - Colostrum is designed to be a perfect source of nutrition and energy for the newborn, and consequently it contains four times as many minerals, five times as much protein, and five to ten times as many vitamins (such as vitamins A, B12, riboflavin, folic acid, and choline) as whole milk. Colostrum is also higher in energy than milk. In addition, colostrum is a major source of high levels of vitamin E and selenium, which are known to confer beneficial anti-oxidant effects. Results of nutritional analysis of PETerna® have shown that our product contains a minimum of 40% crude protein, a minimum 20% crude fat, and a maximum of 1% crude fibre.

 

4.  Other Important Factors

 

Alpha-lactalbumin acts as a co-enzyme in the synthesis of lactose and is also inmunomodulatory, enhancing interleukin-1 production and increasing the metabolic activity of the cells of the immune system (Wong, et. al. 1997 Vet. Immunol. Immunopathol. 56: 85 – 96).

 

Trypsin inhibitor prevents the proteolytic degradation of proteins in the digestive tract and enhances the bioavailability of co-administered molecules. The presence of this component may protect other important factors from digestive degradation (Quigley, J. D. et. al. Supra).

 

Proline Rich Polypeptides have been shown to improve cognitive function - Improved mental and cognitive function demonstrated in people and rats (Behav. Brain Res. 2001;118:201-208)

 

 

Pets Enjoy the Taste of PETerna®

 

Palatability trials have shown that dogs enjoy the taste of PETerna®.  In one trial, each of 30 dogs (convenience sample) was offered its regular ration of commercial dog food, or its regular ration sprinkled with 5 grams (one teaspoon) of PETerna® per imperial cup of dog food.  Ninety-six percent (29/30) of the dogs readily consumed the PETerna®-supplemented ration, and forty-one percent (11/30) demonstrated a clear preference for it.

 

Owners of twenty-six out of the 30 dogs enrolled in the above trial agreed to maintain their dogs in the trial for 30 days and to record all perceived changes over this time period.  Dogs in this trial phase were fed PETerna® as a nutritional supplement daily for 30 days, at the dose of 5 grams (one teaspoon) per day. Even though this was a trial of short duration, some owners were able to observe changes and reported noticeable beneficial effects.  Overall, 33% of the dog owners reported benefits:

 

Three owners reported a clear improvement in the appetite of their dogs.

Five reported a clear improvement in hair coat quality.*

One reported improved consistency of feces.

 

None of the 26 owners reported any adverse reactions to PETerna® after 30 days of feeding. Longer-term studies are being conducted.

*Diminished body odor was frequently reported

  

Manufacturing and Safety

 

PETerna® is manufactured by the Saskatoon Colostrum Company Ltd. in CFIA (Canadian Food Inspection Agency)-inspected facilities using approved outlines of production and quality assurance protocols. The product contains only colostrum collected after calving from Western Canada dairy cows using the same standards as for milk for human consumption. It is heat treated to ensure pasteurization. This is followed by micro-biological testing to ensure that there is no disease causing bacteria. PETerna®  has demonstrated its safety in several animal trials.

 PETerna® “Defined” Composition is Based on Strict Quality Control

 

Figure 1 shows an example of the Quality Control tests performed to assure that PETerna® contains specified levels of the key factors described above.  In this example, the levels of IGF-1 were measured in liquid colostrum before processing and in PETerna® after processing in two different production serials. The results confirm that the pasteurization equivalent heat treatment used, which ensures that pathogenic bacteria are killed, and the spray drying process used to produce PETerna® powder, have no significant impact on the concentration of this important ingredient.  Similar assays are performed to confirm the levels of all other important components of this natural product.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Selected list of references:

 

Carpenter, G. (1980).  Epidermal growth factor is a major growth-promoting agent in human milk.  Science, 210, 198-199.

 

Korhonen, H., P. Marnila, and H.S. Gill, (2000). Milk immunoglobins and complement factors. Br. J. of Nut. 84, Suppl. 1, S75- S80.

 

Pakkanen, R. and J. Aalto, (1997). Growth factors and antimicrobial factors of bovine colostrum. Int. Dairy J.7, 285 - 297. 

 

Playford, R.  J., C. E. Macdonald, and W. S. Johnson, (2000). Colostrum and milk-derived peptide growth factors for the treatment of gastrointestinal disorders. Ann. J. of Clin. Nutr.  72, 5 – 14.

 

Playford, R.J., C.E. Macdonald, D.P. Calnan, D. N. Floyd, T. Podas, W. Johnson, A.C. Wicks, O. Bashir, and T. Marchbank. (2001). Co-administration of the health food supplement, bovine colostrum, reduces the acute non-steroidal anti-inflamatory drug-induced increase in intestinal permeability. Clin. Sci. 100, 627-633.

 

Rona, Z. P. (1998) Bovine colostrum, immunity, and the aging process. Nature’s Impact August/September 57 – 59.

 

Shah N.P. (2000). Effect of milk-derived bioactives: an overview. Brit. J. of Nut., 84, Suppl. 1, S3-S10.

 

Steijns, J.M. and van Hooijdonk A.C.M. (2000).  Occurrence, structure, biochemical properties, and technological characteristics of lactoferrin.  Brit. J. of Nut., 84, Suppl. 1, S11-S17.

 

Taillon, C., and Andreasen, A.  (2000). Veterinary nutraceutical medicine. Can. Vet.  J.  41, 231 – 234.

 

Van Hooijdonk A.C.M., K.D. Kussendrager, and J.M. Steijns, (2000). In vivo antimicrobial activity of components in bovine milk and colostrum involved in non- specific defense.  Brit. J. of Nut., 84, Suppl. 1, S127-S134.

 

For further information call Colleen Tabbernor at (403)949 3119
 PETerna  is a registered trademark of the Laboratory for Advanced
Colostrum Technologies LLC

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